The following information was compiled from the Material Safety Data Sheets and Product Information Sheets supplied with the fluo-3/AM dye from Molecular Probes, Inc. It includes information necessary for determining fluo-3 solution concentrations and information regarding optical absorption and fluorescence wavelengths.

fluo-3/AM Material Safety Data

Product Identity Information

Molecular Formula: C₅₁H ₅₀C_l2N₂ O₂₃

Molecular Weight: 1129.86

CAS Number/Name: 121714-22-5 Glycine, N-[4-[6-[(acetyloxy)methoxy]- 2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxyethyl]amino]-5-methylphenoxy]ethoxy]phenyl]- N-[2-[(acetyloxy)methoxy]-2-oxyethyl]-,(acetyloxy)methyl ester

Physical/Chemical Characteristics

Appearance: Orange Solid

Solubility in Water: Low

Fire and Explosion Hazard Data

Flash Point: Not determined

Unusual Hazards: No unusual fire or explosion hazards.

Extinguishing Fire: Use carbon dioxide, dry chemical extinguishers or water. An approved self-contained breathing apparatus and protective clothing are recommended.

Reactivity Data and Storage Conditions

Stability: Stable. Not a significant hazard in these quantities.

Storage Conditions: Freezer storage recommended. Desiccation recommended. Protect material from light, especially in solution.

Health Hazard Data

Routes of Entry: May enter the body through inhalation, ingestion, and eye and skin contact.

RTECS Number: None known

Exposure Limits: Not determined

Toxicity: We are not aware of any toxicity data for this product.

Health Hazards: We are not aware of any reported health hazards for this product. We recommend treating all chemicals with caution.

Potential Hazards: To our knowledge, the health hazards have not been thoroughly investigated.

Carcinogenicity: Not listed by NTP, IARC or OSHA.

Exposure Symptoms: Unknown. Handle with care.

First Aid: Potentially harmful; avoid prolonged or repeated exposure. Wash thoroughly after handling. If eye or skin contact occurs, wash affected areas with water for 15 minutes and seek medical advice. If inhaled, move individual to fresh air and seek medical advice. If swallowed, seek medical advice.

Precautions for Safe Handling and Use/Control Measures

Ventilation: Mechanical ventilation and respiratory protection are recommended.

Handling: Gloves, protective clothing and eyewear should be worn and safe laboratory practices followed.

In Case of Spill: Use appropriate protective equipment and methods to clean up spilled substance promptly. Absorb spill onto an appropriate material. Collect and dispose of all waste in accordance with applicable laws.

Clean-up: Wash with acetone and/or alcohol, then soap and water.

Data from Product Information Sheets for fluo-3/AM

Spectral Response

l_EX: 506 nm (peak absorption)

l_EM: 526 nm (peak emission)

Quantum Yield, No Ca²⁺ Present: 0.0025

Quantum Yield, Ca²⁺ Present: 0.183

FLuorescence Icrease upon Ca2+ Binding (F_max /F_min): >80

K_D: 320 nM (molar dissociation constant)

fluo-3/AM Bathing Solution Composition

Mix (in order):

50 mg fluo-3/AM (dry, frozen)

20 mL DMSO (dry)

20 mL 20% (weight/volume) Pluronic (F-127) in DMSO

3 mL TES Ringer's Solution

Total Solution Volume: 3.04 mL

Total fluo-3/AM: 50 mg = 44.2 nmol

fluo-3/AM Concentration: 14.55 mM